Table 1.
Cytokine production of splenocytes stimulated by soluble tachyzoite antigens of T. gondii (STAg) evaluated by ELISA
| Groups (n=3) | Cytokine productiona (pg/ml) | ||||
|---|---|---|---|---|---|
| IL-4 | IL-10 | IL-12 (p70) | IFN-γ | TNF-α | |
| Blank | 13.0±2.3 | ND | ND | 34.4±1.9 | 19.5±3.2 |
| PBS | 12.2±3.5 | ND | ND | 33.6±2.6 | 20.7±2.2 |
| pDsRed2-N1 | 14.6±1.4 | ND | ND | 33.1±3.5 | 22.1±3.6 |
| pDsRed2-GRA8 | 15.8±2.7 | 27.5±5.4*** | 32.5±4.1*** | 105.2±6.8*** | 65.4±6.3*** |
a
Values for IL-4, IL-10, IL-12 (p70), IFN-γ, and TNF-α are at 24, 72, 96, 96, and 48 hr, respectively. ND=non-detectable. Data represent the means±SD from 3 mice per group. Three independent experiments were performed, and the data from 1 representative experiment are shown.
***
P<0.001 compared with the blank, PBS and pDsRed2-N1 empty vector groups.