Figure 2.

IFN-Stimulated Nucleosome Reorganization

Genome browser diagram of IFN-induced ISGF3 recruitment and nucleosome dynamics at select ISGs with (A) high to (B) moderate to (C) low or no nucleosome loss shown within 2000 bp +/− TSS. (A–C) (Top) 5′ End of gene depicted with the black arrow depicting the direction of transcription; the small and large black bars representing untranslated and exonic regions, respectively; and the line representing intronic regions. (Middle) ChIP-seq density of STAT1, STAT2, and IRF9 occupancy after mock or 2-hr IFNα treatment in HeLa cells. (Bottom) Nucleosome occupancy after mock, 2-hr, 6-hr or 10-hr IFNα treatment in HeLa cells. Red arrows highlight nucleosome loss at ISGF3-ISRE proximal regions. Red bars beneath nucleosome maps denote nucleosome loss due to 2, 6, or 10-hr IFNα treatment compared with mock (Poisson p value ≤ 1 × 10⁵). All sequencing reads are normalized to 10 million reads.

See also Table S2.