Figure 3.

IFN-Stimulated Loss of Histones H2A.Z, H2B, H3, and H4 at ISG Promoters

(A–F) ChIP analysis of histones H4, H3, H2B, H2A, and H2A.Z occupancy at the gene body (A–C) or gene promoter (D–F) of OAS3, IFIT1/ISG56, IFITM1/9–27 during steady state and after 2-hr IFNα stimulation. The position of the gene body and promoter-specific primers and their relative distance are indicated in the upper panel of A–C. Error bars denote mean ± SD of three technical replicates from one representative experiment. Statistical analysis was computed using the Student's t test with n ≥ 2 (*p < 0.05, **p < 0.005).