Figure 4.
Histone Variant, H2A.Z, Is a Dynamic Component of ISG Promoters
(A) Heatmap depicting steady-state and IFNα-recruited STAT2 occupancy (in-house ChIP-seq) compared with steady-state H2A.Z, H3K4me3, and H3K27me3 occupancy (ENCODE ChIP-seq) at the top 250 enriched STAT2 target loci spanning ±2,500 bp from the STAT2 peak center. H2A.Z and STAT2 occupancy at these loci had a Pearson correlation coefficient of R2 = 0.83.
(B) Genome browser view of H2A.Z occupancy at steady state and STAT2 occupancy after 2-hr IFNα stimulation at three ISGs, OAS3, IFIT1/ISG56, and IFITM1/9–27.
(C) ChIP analysis of H2A.Z removal and recovery after 3-hr IFNα treatment followed by recovery without IFNα for 0 hr or 8 hr at the OAS3, IFIT1/ISG56, and IFITM1/9–27 promoters. Error bars denote mean ± SD of a representative experiment with three technical replicates.
(D) ChIP analysis of steady-state and IFN-stimulated H3K4me3 at OAS3 and IFIT1/ISG56 promoters. Error bars denote mean ± SD of a representative experiment with three technical replicates.
See also Table S1.