Figure 3.

Three-Dimensional Analysis of Reconstructed Patient Mitochondria Reveals Abnormal Morphology and Distribution of Cristae, Related to Videos S3 and S4

(A) Representative three-dimensional reconstruction of in situ mitochondria in tomograms from control and patient fibroblasts. The latter demonstrate abnormally bloated cristae.

(B) Quantitation of the surface area-to-volume ratio of total cristae membrane (light green) surface area to intracristal space (yellow) volume; * indicates p = 0.03.

(C) Quantitation of crista content, expressed as total cristae volume (membrane [light green in (A)] + intracristal space [yellow in (A)]) per matrix volume [gray in (A)]; * indicates p = 0.02.

(D) Quantitation of crista lamellarity, expressed as the ratio of surface area of the crista membrane to the crista lumen volume. In all calculations, we examined n = 10 mitochondria for control and n = 10 for patient; * indicates p = 0.02. For all graphs, significance was tested by the Mann-Whitney test at 95% confidence. Data were collected from at least three independent cells grown on three independent grids for both patient and control.

* indicates statistical significance.