Figure 2. Attachment and growth of ARPE-19 cells with or without an underlying fibronectin matrix.

We tested effects of an extracellular matrix (ECM) such as fibronectin on the ability of cells to form a monolayer on transwell membranes. Inserts coated with fibronectin [ A, C, E] or those without any coating [ B, D, F] were imaged over several days after seeding. No differences were observed in cell attachment or growth. Contact inhibition proceeded cell differentiation after approximately 10 days in culture. Scale bars correspond to 200μm.