Figure 3. A convenient procedure for removing and sectioning membrane inserts from transwells.

Transwell membranes can be detached by making incisions with a scalpel following the circumference of the plastic chamber. The insert lip may be used as an indicator of the boundary. Once removed, the detached membrane can be guillotined using a sharp razorblade. We advise against slicing or cutting using sheer forces as this is likely to damage or dislodge the Retinal Pigment Epithelial monolayer.