Table 1. Materials used to prepare optimised ARPE-19 culture medium.

Product	Product Reference	Supplier	Volume (ml)	Storage
DMEM, high glucose, pyruvate	41966-029	Life Technologies, UK	245	4°C
Heat inactivated new born calf serum (FCS)	N4762	Sigma Aldrich, UK	2.5	Short term: 4°C Long term: -20°C Note: Avoid freeze-thaw of FCS by preparing aliquots.
Penicillin-Streptomycin	P4333	Sigma Aldrich, UK	2.5	-20°C

Abbreviations: Dulbecco’s Modified Eagle Medium (DMEM)