Table 2. Coating volumes for extracellular matrix proteins such as fibronectin used in transwell inserts of different diameters.
Culture surface | Growth Surface
Area (cm 2) |
Coating
Volume (ml) |
Wash
Volume (μl) |
---|---|---|---|
Corning
® 24mm, 0.4μm pore, PET
Transwell ® Permeable Support |
4.67 | 0.6 | 1 |
Corning
® 12mm, 0.4μm pore, PET
Transwell ® Permeable Support |
1.12 | 0.25 | 0.4 |
Abbreviations: Polyethylene terephthalate (PET)