Table 4. Media volumes and feeding regimes used for the culture of ARPE-19 cells in transwell inserts.
Media changed every 2–3 days.
| Transwell size | Volume in each transwell
compartment (ml) |
Volume change (%) |
|---|---|---|
| Corning
® 24mm, 0.4μm pore, PET
Transwell ® Permeable Support |
Upper chamber: 2
Lower chamber: 3 |
Upper chamber: 100
Lower chamber: 20 |
| Corning
® 12mm, 0.4μm pore, PET
Transwell ® Permeable Support |
Upper chamber: 0.5
Lower chamber: 1.5 |
Upper chamber: 100
Lower chamber: 20 |
Abbreviations: Polyethylene terephthalate (PET)