Table 5. List of primary antibodies used for confocal immunofluorescence studies.
| Primary antibody | Supplier | Product
number |
RRID | Host
species |
Clone/isotype | Dilution |
|---|---|---|---|---|---|---|
| Zonula Occludens-1 (ZO-1) | Thermo Fisher
Scientific |
40-2200 | AB_2533456 | Rabbit | pAb/IgG | 1:100 |
| Occludin | Thermo Fisher
Scientific |
71-1500 | AB_2533977 | Rabbit | pAb/IgG | 1:100 |
| Retinal Pigment Epithelium-
specific 65 kDa protein (RPE65) |
Abcam | ab78036 | AB_2181006 | Mouse | mAb, IgG1 | 1:100 |
| alpha 1 Na +/K + ATPase | Abcam | ab7671 | AB_306023 | Mouse | mAb, IgG1
Kappa |
1:100 |
| Rab 5 | Abcam | ab18211 | AB_470264 | Rabbit | pAb/IgG | 1:200 |
| Rab 7 | Abcam | ab137029 | AB_2629474 | Rabbit | mAb/IgG | 1:200 |
| LAMP1 | Abcam | ab24170 | AB_775978 | Rabbit | pAb/IgG | 1:1000 |
| LAMP2A | Abcam | ab18528 | AB_775981 | Rabbit | pAb, IgG | 1:1000 |
| LC3B | Abcam | ab48394 | AB_881433 | Rabbit | pAb/IgG | 1:200 |