TABLE 2.
Primer pairs used for quantitative real-time PCR to assess steady-state mRNA level of target genes
| Gene | GenBank accession no. | Sense | Antisense |
|---|---|---|---|
| Claudin-3 | NM_009902.4 | TTTTCCTGTTGGCGGCTCTG | CGAGGTTTCTTTGTCCATTCGG |
| Claudin-11 | NM_008770.3 | ATGGTAGCCACTTGCCTTCAG | AGTTCGTCCATTTTTCGGCAG |
| Occludin | NM_008756.2 | AAGAAAGATGGATCGGTAT | GGAGGCATGTCCTGTG |
| ZO-1 | NM_009386.2 | GCCCTCCGATCATTCC | TTAGACATTCGCTCTTCCTC |
| Claudin-1 | NM_016674.4 | CTTCTGGGTTTCATCCTGG | CAGATTCAGCAAGGAGTCG |
| Claudin-5 | NM_013805.4 | GCCAACATCGTAGTCCG | GTTCTTCTTGTCGTAATCGCC |
| N-cadherin | NM_007664.5 | CATGCTGAGCCACAGTACC | CGCTACTGGAGGAGTTGAGG |
| Lin28 | NM_145833.1 | AGACCAACCATTTGGAGTGC | AATCGAAACCCGTGAGACAC |
| PLZF | NM_001033324.2 | ATTTACTGGCTCATTCAGCG | CCAGTATGGGTCTGTCTGTG |
| Stra8 | NM_009292.1 | ACAAGAGTGAGGCCCAGCAT | CCTCTGGATTTTCTGAGTTGCA |
| Sycp3 | NM_011517.2 | ATGATGGAAACTCAGCAGCAAGAGA | TTGACACAATCGTGGAGAGAACAAC |
| Clgn | NM_009904.3 | CCAGGGTGTTGGACTATGTTTG | CCCCGAGGAAGGTTCATCTTTA |
| klf6 | NM_011803.2 | ACCCGACATGGATGTGCTCCCAAT | GCAGGGCTCACTCTGAAGATA |
| RhoA | NM_016802.5 | CCAAATGTGCCCATCATCCTAGTTG | TCCGTCTTTGGTCTTTGCTGAACAC |
| RhoB | NM_007483.2 | ATATTAGCGTGGGCACCGAG | GTAGGGGTGTAGGGAGTCGT |
| Cdc42 | NM_026514.2 | CCTTCCACAGCTCAGAGAAA | GCACCAGCGAGGACTGTT |
| Gapdh | NM_008084.3 | TGTGTCCGTCGTGGATCTGA | CCTGCTTCACCACCTTCTTGA |