Figure 9.

Compound 1 increases glycolysis and decreases O₂ consumption in muscle tissue from ND- and HFD-fed mice. A–C) PFK (A), PK (B), and LDH (C) activities were determined in muscle tissue lysates (50 μg protein) from the differently fed and treated animals. D, E) ATP and AMP levels were measured in the same extracts (D) and their ratio was calculated (E). F) O₂ consumption was induced by the addition of pyruvate/malate and blocked by rotenone, the specific inhibitor of complex I. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.