Table 3. Yield of protein, Yield of protease inhibitor activity and Fold of purification in comparison with crude extract.

Purification method	Volume (ml)	Total protein (mg)	Total activity (U)	Specific activity (U/mg)	Yield of protein	Yield of activity	Fold purification
Extract	200	880	90240	102.54	100*	100*	1*
(NH4)2SO4	10	52	7315	140.67	6	8.106	1.37
CM cellulose	10	12	8098	6749	0.136	7.47	65.81