Figure 2.

Effect of irradiation on podocyte cytoskeleton. A) Podocytes were irradiated at 0 Gy [control (cn)] or 8 Gy and morphological changes were studied by laser scanning confocal microscopy after 2 h. F-actin is labeled with rhodamine phalloidin (red), and actin-binding protein ezrin is labeled by FITC-conjugated antibody (green) and nuclei with DAPI (blue). Original magnification, ×40. Scale bars, 20 µM. Representative areas are enlarged in the insets. B) The percentage of cells with cytoskeletal remodeling was obtained by averaging cells with altered actin and ezrin staining per 100 cells. Results are representative of at least 3 independent experiments.