Figure 6.

RTX pretreatment mitigates radiation-induced podocytopathy. A) Podocytes were irradiated (8 Gy) in the presence of RTX (100 µg/ml) or IgG (100 µg/ml). B) SMPDL3b protein expression and cleaved caspase-3 were assayed by immunoblotting. Values represent means ± sem. *P < 0.05. C) Immunofluorescence staining of podocytes after 8 Gy radiation in the presence of RTX (100 µg/ml) or IgG (100 µg/ml) antibody. Actin fibers are labeled with rhodamine phalloidin (red), actin binding protein ezrin with FITC-conjugated antibody (green), and nuclei with DAPI (blue). Original magnification, ×40. Scale bar, 50 µM.