Figure 8.

Down-regulation of SMPDL3b in irradiated murine kidneys. WT C57BL6 mice were exposed to focal bilateral kidney irradiation (14 Gy). After 11 wk, kidneys were extracted and frozen. A) Immunofluorescence staining of paraffin-embedded renal tissues with SMPDL3b (FITC, green), WT-1 (red), and nuclei (DAPI) after 0 Gy [control (cn)] and 14 Gy irradiation. B) Isolates from irradiated and control kidneys were tested for SMPDL3b protein levels via Western blotting. Statistically significant differences were determined using Student's t test. A value of P < 0.05 was considered significant.