Somatodendritic Redox State and ATP/ADP Ratio before and after mt-KR Induced Oxidative Insult in Primary Hippocampal Neurons
(A–E) Fluorescence ratio (405 nm/488 nm) of the matrix-roGFP protein before (A), 30 s after (B), and 30 min after (C) mt-KR activation. For each representative image, a zoomed-in image is shown (400%). (D and E) Quantification of the signal in different neuronal compartments. Values represent the mean ± SEM of 19 cells from 3 independent hippocampal cultures.
(F–J) Fluorescence ratio (405 nm/488 nm) of the cytosolic roGFP protein before (F), 30 s after (G), and 30 min after mt-KR activation (H). For each representative image, a zoomed-in image is shown (400%). (I and J) Quantification of the signal in different neuronal compartments. Values represent the mean ± SEM of 11 cells from 3 independent hippocampal cultures.
(K–O) Fluorescence ratio (488 nm/405 nm) of the Perceval protein (ATP/ADP ratio) before (K), 30 s after (L), and 30 min after mt-KR activation (M). For each representative image, a zoomed-in image is shown (400%). (N and O) Quantification of the signal in different neuronal compartments. Values represent the mean ± SEM of 11 cells from 3 independent hippocampal cultures.
(E, J, and O) One-way ANOVA and Dunnett's multiple comparisons test versus baseline (before mt-KR). *p < 0.05; **p < 0.01; ****p < 0.0001. mt-KR: mito-KillerRed.