Figure 3.
Somatodendritic Redox State and ATP/ADP Ratio after Focal mt-KR Activation in the Soma or the Dendrites
(A) For analysis of events after focal soma stimulation, neuronal images were divided into the stimulated region of interest (ROI), representing a 10 μm2 area; the area directly surrounding the stimulated ROI; the soma; the proximal dendrites; and the distal dendrites.
(B–D) Mitochondrial redox state (B), cytosolic redox state (C), and ATP/ADP ratio (D) in different neuronal compartments after focal mt-KR activation in the soma. Values represent the mean ± SEM of 10–11 cells from three independent hippocampal cultures. Statistics are detailed in Table S1, and the corresponding images are displayed in Figure S1. The dashed line indicates the time of mt-KR activation.
(E) For the analysis of events after focal mt-KR activation in dendrites, neurons were divided into the stimulated ROI, representing a 10 μm2 area; the area directly surrounding the ROI (around ROI 1); the rest of the stimulated dendrite (around ROI 2); the soma; the non-stimulated proximal dendrites; and the non-stimulated distal dendrites.
(F–H) Mitochondrial redox state (F), cytosolic redox state (G), and ATP/ADP ratio (H) in different neuronal compartments after focal mt-KR activation in the dendrite. Values represent the mean ± SEM of 14–16 cells from 3 independent hippocampal cultures. Statistics are detailed in Table S1, and the corresponding images are displayed in Figure S2. The dashed line indicates the time of mt-KR activation.