Figure 1.
Kinetics of amyloid fibril formation measured by ThT fluorescence. Representative ThT kinetics curves for H2O2-wt-ApoA-I, MPO-3:1-H2O2-wt-ApoA-I, MPO-10:1-H2O2-ApoA-I, and nonoxidized wt-ApoA-I. A portion of fibrillation incubation mixture was combined with the ThT stock solution at the indicated time points and the fluorescence emission spectrum of the sample recorded. Solid lines are fitting of the experimental values by exponential (H2O2-wt-ApoA-I and MPO-10:1-H2O2-ApoA-I) or logarithmic (MPO-3:1-H2O2-wt-ApoA-I) curves. T1/2 data (means ± sem) from ≥3 independent experiments are shown.