Figure 10.
Seeding of nonoxidized wt-ApoA-I and 90C-wt-ApoA-I with molar 10% of preformed H2O2-wt-ApoA-I aggregates. In control experiments, nonoxidized wt-ApoA-I and 90C-wt-ApoA-I were incubated in the absence of seeds. Total protein concentration was 1.0 mg/ml. The samples were incubated in fibrillation buffer (pH 6.0) at 37°C with continuous vortexing at 1100 rpm. ThT fluorescence was measured at the indicated time points. Solid lines show fitting of the experimental values by monomolecular growth exponential curves. T1/2 data (means ± sem) from ≥3 independent experiments are shown.