TABLE 2.
WMF of monomeric ApoA-I samples at 50 µg/ml in iPBS
| Sample name | WMF (nm) |
|---|---|
| wt-ApoA-I | 339.6 ± 0.2 |
| 90C-wt-ApoA-I | 353.3 ± 0.5 |
| 90C-wt-ApoA-I+20C 1h | 342.0 ± ND |
| MPO-3:1-H2O2-wt-ApoA-I | 342.8 ± 0.2 |
| MPO-10:1-H2O2-wt-ApoA-I | 372.5 ± 0.4 |
| H2O2-wt-ApoA-I | 342.9 ± 0.4 |
| 90C-H2O2-wt-ApoA-I | 353.7 ± 0.3 |
| 90C-H2O2-wt-ApoA-I+20C 1h | 345.0 ± ND |
The excitation wavelength was 280 nm. 90C-wt-ApoA-I+20C 1h, 90C-H2O2-wt-ApoA-I+20C 1h: wt-ApoA-I, and H2O2-wt-ApoA-I samples, respectively, heated at 90°C for 1 h and then incubated at 20°C for 1 h before fluorescence analysis. Full spectra are reported in Supplemental Fig. S1. ND, not determined.