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. 2018 Jan 17;32(6):3149–3165. doi: 10.1096/fj.201701127R

TABLE 2.

WMF of monomeric ApoA-I samples at 50 µg/ml in iPBS

Sample name WMF (nm)
wt-ApoA-I 339.6 ± 0.2
90C-wt-ApoA-I 353.3 ± 0.5
90C-wt-ApoA-I+20C 1h 342.0 ± ND
MPO-3:1-H2O2-wt-ApoA-I 342.8 ± 0.2
MPO-10:1-H2O2-wt-ApoA-I 372.5 ± 0.4
H2O2-wt-ApoA-I 342.9 ± 0.4
90C-H2O2-wt-ApoA-I 353.7 ± 0.3
90C-H2O2-wt-ApoA-I+20C 1h 345.0 ± ND

The excitation wavelength was 280 nm. 90C-wt-ApoA-I+20C 1h, 90C-H2O2-wt-ApoA-I+20C 1h: wt-ApoA-I, and H2O2-wt-ApoA-I samples, respectively, heated at 90°C for 1 h and then incubated at 20°C for 1 h before fluorescence analysis. Full spectra are reported in Supplemental Fig. S1. ND, not determined.