TABLE 1.

Kinetic parameters for Nb–rhMIF interactions as determined by SPR

Family	Nb clone	Kon (105 M−1 s−1)	Koff (103 s−1)	KD (nM)
I	NbB5	0.41	1.54	37.75
II	NbC10	0.38	1.61	41.19
III	NbA2	3.11	0.92	2.960
	NbD2	0.75	1.24	16.50
	NbD4	0.44	1.53	35.00
	NbF10	ND	ND	ND
IV	NbH5	0.17	0.48	27.50
V	NbE5	0.78	0.74	9.410
	NbH9	0.32	2.57	80.30
VI	NbD12	0.18	2.98	166.0
VII	NbE10	0.98	6.32	64.50

Data were measured in the format of a multicycle experiment, with rhMIF as the ligand (i.e., coated onto the sensor surface) and anti-MIF Nbs as the analyte. Experimental data were analyzed with a 1:1 Langmuir binding model to obtain the association rates (K_on), dissociation rates (K_off) and affinity constants (K_D).