Table 2.
Summary of Tau and Phosphorylated Peptides and Half-Lives in the Cell Lysates of iPSC-Derived Neurons
| Peptide (Abbreviated) | Peptide (Full) | Domain | Ami no Acids | Half-Life (Days) | N | |
|---|---|---|---|---|---|---|
| Mean | SD | |||||
| 0N | AEEAGIGDTPSLEDEAAGHVTQAR | N term | 103–126 | 7.23 | 1.01 | 4 |
| p0N | AEEAGIGDT*PS*LEDEAAGHVT*QAR (any) | N term | 103–126 | 6.00 | 0.29 | 4 |
| TPSL | TPSLPTPPTR | Mid | 212–221 | 8.13 | 0.98 | 4 |
| pTPSL 212/214 | T*PS*LPTPPTR (either) | Mid | 212–221 | 6.64 | 0.51 | 4 |
| pTPSL 217 | TPSLPT*PPTR | Mid | 212–221 | 4.96 | 0.36 | 4 |
| TPSL REPK | TPSLPTPPTREPK | Mid | 212–224 | 7.97 | 0.35 | 4 |
| pTPSLREPK 212 | T*PSLPTPPTREPK | Mid | 212–224 | 5.33 | 1.84 | 4 |
| pTPSLREPK 214 | TPS*LPTPPTREPK | Mid | 212–224 | 6.43 | 1.27 | 4 |
| pTPSLREPK 217 | TPSLPT*PPTREPK | Mid | 212–224 | 5.43 | 0.28 | 4 |
| IGST | IGSTENLK | MTBR-N | 260–267 | 6.88 | 0.88 | 4 |
| pIGST | IGS*T*ENLK (either) | MTBR-N | 260–267 | 4.30 | 0.32 | 2 |
| IGSL | IGSLDNITHVPGGGNKK | MTBR-C | 354–369 | 5.90 | 0.33 | 2 |
| pIGSL | IGS*LDNIT*HVPGGGNKK (either) | MTBR-C | 354–369 | 2.39 | N/A | 1 |
| DTSPR | DTSPRHLSNVSSTGSI | Cterm | 402–416 | 4.93 | 0.51 | 2 |
| pDTSPR | DT*S*PRHLS*NVS*S*TGSI (any) | Cterm | 402–416 | 4.92 | 1.31 | 2 |
| Mean unphosphorylated | 6.84 | 1.24 | ||||
| Mean phosphorylated | 5.16 | 1.28 | ||||
N/A, not available due to limit of detection; MTBR, microtubule-binding region; SD, standard deviation; N represents number of cell lines analyzed; asterisk (*), phosphorylation.