Extended Data Figure 1 |. Inferred MHC binding affinities of mutant versus wildtype peptides.

Neoantigens used in this study are 9-residue long peptides affinities predicted to be less than 500nM by NetMHC3.4¹⁹ (SI). We plot predicted affinities of mutant peptides, designated$K_d^{MT}$, versus the predicted dissociation affinities of the wildtype peptides, which generated them, designated$K_d^{WT}$. A single point mutation can lead to predicted dissociation constant difference of up to 4 orders of magnitude.