Figure 1.

Developing the Adipocyte Flow Cytometry Protocol

(A) Freshly isolated adipocytes and SVF cells from AdTomato and WT mice, stained with fluorescein isothiocyanate (FITC)-labeled lectin (green) and Hoechst (blue) and imaged for tdTomato expression (red). Scale bars, 100 μm; note the differences in length.

(B) Flow cytometry using standard settings for scWAT adipocytes and samples containing only pure mouse lipid or sunflower seed oil. Adipocyte samples show ungated and tdTomato⁺ (T⁺) gated events.

(C) Flow cytometry using optimized adipocyte flow cytometry settings for scWAT adipocytes, BAT adipocytes, or SVF cells, showing ungated and tdTomato⁺ (T⁺) or Hoechst⁺ (H⁺) gated events.

All of the experiments were repeated at least four times. See also Figures S1 and S2.