Figure 7.

Flow Cytometry Analyses of ADRB2 Expression in Human Adipocytes

(A) Flow cytometric analysis of paired human omWAT and scWAT adipocyte samples unstained or stained with anti-ADRB2 antibody conjugated to APC. Samples are arranged from left to right in order of increasing patient HOMA-IR index.

(B) Analysis of the percentage of ADRB2⁺ cells in paired scWAT and omWAT adipocyte samples.

(C) Correlation of the percentage of scWAT ADRB2⁺ cells with patient fasting S-insulin levels.

(D) Comparison of the percentage of scWAT adipocytes expressing ADRB2 between metabolically healthy (HOMA-IR index <2.5) and metabolically unhealthy obese patients. Lines shows means ± SDs. ^∗∗∗∗p < 0.0001. See also Figure S7.

(E) Human adipocytes unstained or stained with anti-ADRB2 antibody conjugated to APC (red; Hoechst in blue). Scale bars, 50 μm.

n = 9 patients.