REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-UCP1 antibody	R&D Systems	Cat#MAB6158; RRID: AB_10572490
Mouse IgG2B Alexa Fluor 647-conjugated Isotype Control antibody	R&D systems	Cat#IC0041R; RRID: AB_2737095
Rabbit polyclonal anti-ADRB2 antibody conjugated to APC	LSBio	Cat#LS-C274967; RRID: AB_2737096
Mouse monoclonal anti-ADRB2 antibody conjugated to Alexa647	Santa Cruz	Cat#SC-81577; RRID: AB_1119478
Mouse monoclonal anti-Actin antibody	Sigma-Aldrich	Cat#A5441; RRID: AB_476744
Chemicals, Peptides, and Recombinant Proteins
CL 316,243	Sigma-Aldrich	C5976; CAS:138908-40-4
Specific synthetic blocking peptide toward anti-ADRB2 antibody LS-C274967	LSBio	Cat#LS-E8815
Critical Commercial Assays
Alexa Fluor 647 antibody labeling kit	ThermoFisher	A20186
Experimental Models: Organisms/Strains
Mouse: Adipoq-CreERt: B6.129-Tg(Adipoq-cre/Esr1∗)1Evdr/J	Jeffery et al., 2014	RRID: IMSR_JAX:024671
Mouse: Ai14 tdTomato: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze	The Jackson Laboratory	RRID: IMSR_JAX:007914
Mouse: UCP1-KO: B6.129-Ucp1tm1Kz/J	Enerbäck et al., 1997	RRID: IMSR_JAX:003124
Oligonucleotides
Tbp (Mm00446971_m1)	ThermoFisher	Cat#4331182
Cd45 (Mm01293577_m1)	ThermoFisher	Cat#4331182
Ucp1 (Mm01244861_m1)	ThermoFisher	Cat#4331182
Thbd (Mm00437014_s1)	ThermoFisher	Cat#4331182
Leptin (Mm00434759_m1)	ThermoFisher	Cat#4331182
Software and Algorithms
FlowJo flow cytometry analysis platform	FlowJo, LLC	https://www.flowjo.com
GraphPad Prism 6	GraphPad Software	https://www.graphpad.com/scientific-software/prism/