Table 1.
Primers used in PCR and RT- PCR reactions.
| Virusa | Primer sequence | Annealing temperature | Amplicon size |
|---|---|---|---|
| BHV-1 GB | F GTA CAC GTT CAA GGC CTA CA R TCG TCT CGC AGC ATT TC |
51 °C | 668 bp |
| BVD | F CAT GCC CTT AGT AGG AC R CTC CAT GTG CCA TGT ACA G |
48 °C | 400 bp |
| BRSV | F CAT CAA TCC AAA GCA CCA CAC TGT C R GCT AGT TCT GTG GTG GAT TGT TGT C |
55 °C | 381 bp |
| BPI-3 | F AGT GAT CTA GAT GATGAT CCA R GTT ATT GAT CCA ATT GCT GT |
47 °C | 328 bp |
| BEV | F ATG GAC AAG AGG TAY GTC GTC GT R GGG CAC ACT CCG GAT TTT CTC C |
55 °C | 450 bp |
a
BHV-1, bovine herpesvirus type 1 or infectious bovine rhinotracheitis virus; BVDV, bovine viral diarrhea virus; BRSV, bovine respiratory syncytial virus; BPIV-3, bovine parainfluenza 3 virus; BEV, bovine enterovirus.