Figure 3.

Colocalization of Cyr61 and cav-1. A) Beas2B cells stained with cav-1 antibody (red) and Cyr61 antibody (green). Merged image (yellow) is an overlay of the previous two images (red and green). Arrow indicates colocalization of Cyr61 and cav-1 as revealed by yellow staining. B) Co-IP was performed with Cyr61 antibody on Beas2B and mouse lung fibroblast lysates. After precipitation by the rabbit polyclonal Cyr61 antibodies, equal amount of Cyr61 was confirmed using a separated goat polyclonal anti-Cyr61 antibody. Western blot analysis with cav-1 monoclonal antibodies showed that cav-1 interacted with Cyr61 in both cells in room air. Hyperoxia attenuated the interaction of Cyr61 and cav-1 in both cells. Imaging and immunoblots were performed 3 similar times.