Bmal1−/− mice develop obesity when fed regular chow or a high-fat diet. A) Hematoxylin and eosin staining of white adipose tissue at 10 wk of age. B, C) Total body fat content of mice fed regular chow, as determined by NMR (B) and direct measurement of perigonadal white fat pad weight (C). D) Total body fat content analysis of mice fed a high-fat diet. E) Growth curve of mice fed a high-fat diet (n=7–10/group). ##P < 0.05; 1-way ANOVA. F, G) Gene expression level of adipogenic factors in white adipose tissue, as determined by quantitative RT-PCR (F; n=5) and Western blot analysis (G; n=3). *P < 0.05, **P < 0.01 vs. WT.