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Enhanced adipogenic differentiation in MEF cells of Bmal1−/− mice. A) Oil-red-O staining of MEF cells (top panels) and quantification (bottom panels). B) Oil-red-O staining (top panels) and phase-contrast images (bottom panels). C) Quantitative PCR analysis of adipogenic marker gene expression at d 0, 3, and 6. D) Bmal1 protein level, as determined by immunoblot analysis (n=3). HET, heterozygote; KO, knockout (homozygote). *P < 0.05, **P < 0.01 vs WT.
