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Components of Wnt pathway are direct target genes of Bmal1. A) ChIP assay of Bmal1 occupation in promoters containing E-boxes. Rev-erbα primers are used as positive controls and TBP primers as negative controls for the analysis. Data are expressed as percentage of input level normalized to IgG control (n=3). B) Circadian expression patterns of Bmal1 target genes in Wnt pathway induced by serum shock in 10T1/2 cells, with CT 0 as the first time point after serum shock (n=3/time point).
