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. 2018 Sep 14;18:193. doi: 10.1186/s12870-018-1407-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Expression and promoter analysis of BnaC4.BOR1;1c in B-efficient and B-inefficient cultivars. a Phenotypic performance of B-efficient cultivars (QY 10 and Ningyou 7) and B-inefficient cultivars (W10 and Bakow) that were cultivated in a hydroponic culture under B-limitation conditions (0.25 μM) for 10 d. b Sequence alignment of BnaC4.BOR1;1c promoters from two cultivars. c A 27-bp insertion in the region from − 451 bp to − 478 bp in the QY10 promoter that harbours four important cis-acting elements predicted using PLACE. Expression of BnaC4.BOR1;1c in (d) shoots and (e) roots from QY10 and W10; the plants were grown in 10 μM boric acid for 7 d, then transplanted in 0.25 μM B to analyse the expression after 1, 3, 12, 24, 48, and 72 h of treatment