Fig. 7.

Effect of minocycline treatment on adult and young Ube3a^tm1Alb mice in F1 hybrid 129S2-C57BL/6J background. a Timeline representing minocycline treatment and behavioral phenotyping of adult Ube3a^m−/p+ mice. b–f Effect of minocycline on adult Ube3a^tm1Alb mice on the behavioral test battery. Wild-type and Ube3a^m−/p+ (Ube3a^tm1Alb) vehicle-treated adult mice: n = 9, 11 (WT = 5 females, 4 males; Ube3a^m−/p+ = 6 females, 5 males), with the exception of the nest building (n = 6, 7). Minocycline-treated wild-type and Ube3a^m−/p+ (Ube3a^tm1Alb) adult mice: n = 10, 11 mice (WT = 6 females, 4 males; Ube3a^m−/p+ = 6 females, 5 males), with the exception of the nest building (n = 6, 6). g Timeline representing minocycline treatment and behavioral phenotyping of young Ube3a^m−/p+ mice. h–l Effect of minocycline on young Ube3a^tm1Alb mice on the behavioral test battery. Wild-type and Ube3a^m−/p+ (Ube3a^tm1Alb) vehicle-treated young mice: n = 21, 17 (WT = 11 females, 10 males; Ube3a^m−/p+ = 7 females, 10 males), with the exception of the nest building (n = 13, 12) and the forced swim test (20, 17). Minocycline-treated wild-type and Ube3a^m−/p+ (Ube3a^tm1Alb) young mice: n = 33, 22 mice (WT = 20 females, 13 males; Ube3a^m−/p+ = 8 females, 14 males), with the exception of the open field (33, 21), the marble burying (33, 21), and the nest building (n = 16, 17). A multivariate repeated ANOVA or a two-way ANOVA was used for statistical comparison in behavioral phenotypes. *p < 0.05 and ***p < 0.001 indicate the effect of genotype. In none of the tests, we observed an interaction of genotype and treatment