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. 2018 Sep 7;9:1006. doi: 10.3389/fphar.2018.01006

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Copyright © 2018 Sarno, Papulino, Franci, Andersen, Cautain, Melardo, Altucci and Nebbioso.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CHBH does not target KDM4A. (A) KDM4 activity evaluated by in vitro enzymatic assay in presence of 10 different concentrations of CHBH. PKF118-310 at 10 μM was used as positive control. (B) Binding between KDM4A and CHBH evaluated by EnSpire label-free biochemical assay. The response was measured in picometers (pm) as the difference in wavelength. Values are mean ± SD of biological triplicates. ^∗∗∗∗p-value ≤ 0.0001, ns p-value > 0.05 vs. control.