Fig. 3.

Phylogenetic tree and analysis of amino acids of Gnp4/LAX2. (A) Phylogenetic relationship among Gnp4 and homologous plant proteins. The tree was generated using the MEGA6.0 program by the neighbor-joining method. (B) Schematics of the Gnp4-L1 and Gnp4-L2 protein groups. The RING finger and RAWUL domains are indicated. (C) The amino acids of RAWUL in Gnp4 and homologous proteins. Alignment was conducted using the Megalign program in the DNASTAR software package. The RAWUL domain is indicated in red, the purple box indicates the PDZ binding motif, and the red stars indicate the amino acids required for its interaction with OsIAA3 and OsIAA17.