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. 2018 Jul 28;69(20):4723–4737. doi: 10.1093/jxb/ery256

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© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Subcellular localization and expression pattern analyses of Gnp4/LAX2. (A) Subcellular location of ProSuper::GFP and ProSuper::Gnp4-GFP in tobacco leaf cells. ProSuper::OST1-mcherry is a mCherry marker localized in the nucleus and cytosol (Ding et al., 2015). Scale bars are 20 μm. (B) GUS staining of various tissues of transgenic plants containing the ProGnp4::GUS construct. I, bud; II, seedling; III, root; IV, leaf; V, stem; VI, sheath; VII, glumes; and VIII, panicles at different stages. (C) qRT-PCR analysis of relative expression levels of Gnp4 in different tissues from wild-type Nipponbare. R, root; S, stem; L, leaf; SH, leaf sheath; and P, panicles at sequential lengths, where the number indicates the panicle length (cm). Data are means (±s.e.m.) (n=3 plants each with three technical repeats).