Skip to main content
. 2018 Jul 28;69(20):4723–4737. doi: 10.1093/jxb/ery256

Fig. 8.

Fig. 8.

Gnp4/LAX2 regulates OsERF142 and OsERF3 expression. (A–D) Relative expression levels of OsERF142 and OsERF3 in Gnp4-overexpression and wild-type Nipponbare (Nip) plants or in osarf25 and wild-type Hwayoung (HY). Data are means (±s.e.m.) (n=3 plants each with three technical repeats). Student’s t-test. **P<0.01. (E, F) ProOsERF142 used in yeast one-hybrid assays showing the binding activity of OsARF25 to the P2 region of ProOsERF142. AuxRE cis-elements are indicated with black triangles. (G) ChIP–qPCR indicating the binding of OsARF25 to the P2 region of ProOsERF142. Data are means (±s.e.m.) (n=3). Student’s t-test: **P<0.01. (H, I) ProOsERF3 used in yeast one-hybrid assays showing no interaction between OsARF25 and fragments of ProOsERF3. (J, K) Effectors, reporter, and internal control used in the transient assays to determine the effect of Gnp4 on transcription of OsERF142. Data are means (±s.e.m.). There were four replicates, each with five technical replicates. Different letters indicate significant differences between means according to LSD tests (P>0.05).