Fig. 6.
Validating the binding of structural genes directly downstream of FaMYB44.2 by EMSA. (A) FaMYB44.2 physically interacts with the FaSPS3 promoter (PFaSPS3). The binding of MBS and CGTCA motif was detected in one experiment while the competitive binding of TGA element was performed separately. (B) EMSA between FaMYB44.2 and the FaCS1 promoter (PFaCS1). The specific binding between FaMYB44.2 and PFaCS1 was validated using different concentrations of cold competitive probes. (C) EMSA showing the interaction of FaMYB44.2 with the FaSUS1 promoter. (D) EMSA showing the interaction of FaMYB44.2 with the FaHXK2 promoter; the binding of G-box and CGTCA motif was detected in one experiment while the binding of MBS, ABRE and TATCCAT elements was performed in another experiment. (E) EMSA showing the interaction of FaMYB44.2 with the FaMDH1 promoter. ABRE, cis-acting element involved in the ABA response; CGTCA motif, cis-acting regulatory element involved in the MeJA response; G-box, cis-acting regulatory element involved in the light response; HSE, cis-acting element involved in the heat stress response; MBS, MYB binding site involved in drought inducibility; TATCCAT, cis-acting element involved in repressing the sugar response with a G-box; TC-rich, cis-acting element involved in defense and stress responses; TGA, auxin-responsive element.