Table 1.
Correlation of expression between the intensities of FL family members fused to YFP and ST–RFP, SYP61–RFP, SYP61–CFP, FKD1–GFP, or 35S:GFP–RABA1c in leaf epidermis of Nicotiana tabacum and in the cotyledon epidermis of Arabidopsis seedlings at 2.5 DAG
| FL family fusion | Marker | n | Tissue | Mean PCC | Frequency of FL family with marker |
|---|---|---|---|---|---|
| pUBQ10:FL1-YFP | 35S:ST–RFP | 28 | N. tabacum | 0.09 ± 0.21 | 0.13 ± 0.06 |
| pUBQ10:FL3-YFP | 35S:ST–RFP | 25 | N. tabacum | 0.05 ± 0.19 | 0.11 ± 0.05 |
| pUBQ10:FL5-YFP | 35S:ST–RFP | 25 | N. tabacum | 0.61 ± 0.20 | 0.91 ± 0.24 |
| pUBQ10:FL6-YFP | 35S:ST–RFP | 22 | N. tabacum | 0.61 ± 0.16 | 0.99 ± 0.01 |
| pUBQ10:FL7-YFP | 35S:ST–RFP | 21 | N. tabacum | 0.17 ± 0.15 | 0.08 ± 0.04 |
| pUBQ10:FL1-YFP | 35S:SYP61–RFP | 30 | N. tabacum | 0.69 ± 0.13 | 0.96 ± 0.03 |
| pUBQ10:FL3-YFP | 35S:SYP61–RFP | 25 | N. tabacum | 0.63 ± 0.09 | 0.98 ± 0.02 |
| pUBQ10:FL3-YFP | SYP61pro:SYP61–CFP | 54 | Arabidopsis | 0.38 ± 0.12 | 0.71 ± 0.11 |
| pUBQ10:FL5-YFP | 35S:SYP61–RFP | 28 | N. tabacum | 0.05 ± 0.13 | 0.11 ± 0.11 |
| pUBQ10:FL6-YFP | 35S:SYP61–RFP | 37 | N. tabacum | –0.04 ± 0.11 | 0.06 ± 0.04 |
| pUBQ10:FL7-YFP | 35S:SYP61–RFP | 24 | N. tabacum | 0.12 ± 0.22 | 0.06 ± 0.06 |
| pUBQ10:FL1-YFP | 35S:FKD1–GFP | 25 | N. tabacum | 0.19 ± 0.25 | 0.22 ± 0.16 |
| pUBQ10:FL3-YFP | 35S:FKD1–GFP | 44 | N. tabacum | 0.37 ± 0.25 | 0.26 ± 0.26 |
| pUBQ10:FL3-YFP | 35S:FKD1–GFP | 25 | Arabidopsis | 0.46 ± 0.26 | 0.25 ± 0.15 |
| pUBQ10:FL5-YFP | 35S:FKD1–GFP | 48 | N. tabacum | 0.20 ± 0.22 | 0.22 ± 0.20 |
| pUBQ10:FL6-YFP | 35S:FKD1–GFP | 27 | N. tabacum | –0.07 ± 0.22 | 0.05 ± 0.06 |
| pUBQ10:FL7-YFP | 35S:FKD1–GFP | 46 | N. tabacum | 0.76 ± 0.21 | 0.87 ± 0.32 |
| pUBQ10:FL1-YFP | 35S:GFP–RABA1c | 22 | N. tabacum | 0.76 ± 0.13 | 0.36 ± 0.09 |
| pUBQ10:FL3-YFP | 35S:GFP–RABA1c | 35 | N. tabacum | 0.75 ± 0.12 | 0.60 ± 0.19 |
| pUBQ10:FL5-YFP | 35S:GFP–RABA1c | 23 | N. tabacum | 0.34 ± 0.19 | 0.21 ± 0.28 |
| pUBQ10:FL6-YFP | 35S:GFP–RABA1c | 23 | N. tabacum | 0.01 ± 0.12 | 0.21 ± 0.00 |
| pUBQ10:FL7-YFP | 35S:GFP–RABA1c | 31 | N. tabacum | 0.68 ± 0.27 | 0.86 ± 0.29 |
Mean PCC was determined using the co-localization plugin in NIH image J from the intensity scatterplot of merged images. Frequency of FL family fusion with each marker was determined by counting the number of punctae labelled by both markers, and dividing it by the number of punctae labelled by the FL family protein