Table 4.
Comparison of secondary vein characteristics (second and third secondary veins) between the wild type (WT) and the fkd1/fl2/fl3 triple mutant
| A. Genotype (n=68 for the WT and n=95 for fkd1/fl2/fl3) | Number of non-meeting secondary veins | Percentage of non-meeting secondary veins | ||
|---|---|---|---|---|
| WT second secondary vein | 0 | 0 | ||
| fkd1/fl2/fl3 second secondary vein | 36* | 18.9 | ||
| WT third secondary vein | 0 | 0 | ||
| fkd1/fl2/fl3 third secondary vein | 60 * | 31.6 | ||
| B. Genotype (n=45 for the WT and n=65 for fkd1/fl2/fl3) | Percentage wide PED | |||
| WT second secondary vein stage I | 10.7 | |||
| fkd1/fl2/fl3 second secondary vein stage I | 30.3* | |||
| WT second secondary vein stage II | 1.1 | |||
| fkd1/fl2/fl3 second secondary vein stage II | 16.0* | |||
| WT third secondary vein stage I | 26.4 | |||
| fkd1/fl2/fl3 third secondary vein stage I | 28.2 | |||
| WT third secondary vein stage II | 10.0 | |||
| fkd1/fl2/fl3 third secondary vein Stage II | 25.2 | |||
| C. Genotype (n=15 for both genotypes) | Percentage PIN1–GFP localization | |||
| Apical | Basal | Lateral | All sides | |
| WT second secondary vein stage I | 7.8 | 40.8 | 51.3 | 0 |
| fkd1/fl2/fl3 second secondary vein stage I | 2.8 | 25.0 | 25.0 | 47.2* |
| WT second secondary vein stage II | 43.3 | 37.8 | 18.9 | 0 |
| fkd1/fl2/fl3 second secondary vein stage II | 13.0* | 19.3* | 14* | 53.3* |
| WT third secondary vein stage I | 3.0 | 56.7 | 40.0 | 0 |
| fkd1/fl2/fl3 third secondary vein stage I | 5.2 | 26.5* | 15.4* | 52.9* |
| WT third secondary vein stage II | 40.9 | 40.9 | 18.1 | 0 |
| fkd1/fl2/fl3 third secondary vein stage II | 15.0* | 18.3* | 19.0 | 47.7* |
(A) Frequency of non-meeting secondary veins, analysed at 21 DAG; (B) wide (three or more cells) PIN–GFP expression domains (PEDs) at stage I and stage II of development; (C) localization of PIN1–GFP within PED cells to different membrane faces at stage I and stage II of development.
*Significant difference (P<0.05) from the wild type; where percentage values are shown, significant differences were calculated from raw data using Fisher’s test.