Table 1.

Primer sequences (forward and reverse) used for real-time PCR

Genes	Primer sequence (5´–3´)	T m (°C)	GC content (%)
IL-1β	F: GCTCTTGGGGTAGACTTTGGGGT R: CCCGAGCCCTGCTGATCCAT	64.2 63.5	56.5 65.0
IL-6	F: TCATTAAGCGCATGGTCGACAAA R: TCAGCTTATTTTCTGCCAGTGTCT	58.9 59.3	43.5 41.7
IL-8	F: CACTGTGAAAATTCAGAAATCATTGTTA R: CTTCACAAATACCTGCACAACCTTC	57.8 61.3	28.6 44.0
IL-12	F: TTATTGAGGTCGTGGTAGAAGCTG R: GGTCTCAGTTGCAGGTTCTTGG	61.0 62.1	45.8 54.5
GM-CSF	F: AGTAATGACACAGAAGTCGTCTCTG R: GCCGTTCTTGTACAGCTTCAGG	61.3 62.1	44.0 54.5
IFN-γ	F: TCATTAAGCGCATGGTCGACAAA R: TCAGCTTATTTTCTGCCAGTGTCT	58.9 59.3	43.5 41.7
TNF-α	F: TCTTCTCAAGCCTCAAGTAACAAGC R: CCATGAGGGCATTGGCATAC	61.3 59.4	44.0 55.0
GAPDH	F: GGCGTGAACCACGAGAAGTATAA R: CCCTCCACGATGCCAAAGT	60.6 58.8	47.8 57.9