FIG. 3.

Test of allele specificity of suppression. Each of the ceg1-ts strains (see Table 1) was transformed with 2μ-URA3 clones of CES1, CES2, and CES3 in parallel with 2μ-CEG1 and YEP24 vector plasmids. Ura+ transformants were streaked to plates lacking uracil. The plates were incubated for 4 days at either 25°C or 37°C. The strength of suppression at 37°C was scored by comparison to the wild-type CEG1 transformant (+ + +) and the YEP24 vector (−), which were included as controls on every culture plate.