FIG. 5.

Identification of the CES1 gene, ceg1-25 was transformed with the original 2μ-CES1 isolate containing a ∼9-kbp insert (CESl-9kb), with a genomic 2μ clone containing a 5-kbp insert that was isolated by colony hybridization (CESl-5kb), with a derivative of CESl-5kb in which the 1-kbp Bg/II fragment was deleted (CESl-ΔBg1), and with a YEP24-based plasmid containing a 3.8-kbp HincII fragment derived from CES1-5kb (CES1-Hinc2) (see Fig. 4). Ura+ transformants were selected and streaked on plates lacking uracil. Wild-type CEG1 and YEP24 vector transformants were streaked on the same plates as controls. The plates were photographed after incubation for 4 days at either 25°C or 37°C.