FIG. 2.

Repression of Thy-1 gene expression in T × L-cell hybrids. (A) Total RNA (15 μg) from parental and hybrid cells were electrophoresed through formaldehyde/agarose gel, transferred to Nytran membranes, and hybridized with labeled Thy-1 cDNA probe. Lanes 1–12: BW5147, L, Hy1A, Hy2A, Hy3A, Hy1B, Hy3B, Hy4B, Hy1C, Hy2C, Hy3C, and Hy4C, respectively. (B) Different amounts of total BW5147 RNA (10, 5, and 1 μg; lanes 1–3, respectively) and poly(A)⁺ RNA of Hy1A (10, 5, and 1 μg; lanes 4–6, respectively) were hybridized with Thy-1 cDNA radioactive probe. The blots were stripped, rehybridized with a β-actin cDNA probe, and shown in the lower parts of this figure.