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. 2018 Sep 14;5(6):285–300.

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Copyright © 1996 Cognizant Comm. Corp.

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FIG. 8 — T × L-cell hybrids express Ets-1 and low levels of GATA-3, LEF-1/TCF-1α, and TCF-1 mRNAs. (A) RNA (15 μg/lane) isolated from BW5147 (lane 1), L cells (lane 2), Hy3A (lane 3), and Hy1B (lane 4) cell lines were electrophoresed on 1% agarose-formaldehyde gel, transferred to Nitran filter, and hybridized sequentially with GATA-3, LEF-1, TCF-1, and β-actin radioactive probes. (B) RNA (15 μg/lane) isolated from parental BW5147 (lane 1), L cells (lane 2), Hy3A (lane 3), and Hy1B (lane 4) cell lines were electrophoresed, blotted, and hybridized with a labeled ets-1 probe.