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DNA mobility shift assays of nuclear extracts from skin, scar, and keloid fibroblasts binding fragments of the human fibronectin promoter. Interactions of nuclear extracts with 32P-labeled A, B, and C fragments are shown. The discrete bands generated by incubation of the A probe with nuclear extracts are numbered 1–4. +: assays performed in the presence of 100-fold molar excess unlabeled probe as competitor.
