FIG. 10.

Effect of bacterial toxins on PAF-induced κB binding activity. The CHO-PAFR cells were pretreated with either cholera toxin (CT) or pertussis toxin (PT) at the indicated concentrations for 4 h in the culture medium. The cells were then stimulated with ligands for 40 min and nuclear extracts were analyzed by EMSA. The concentrations of the toxins used with TNF-α were 2 μg/ml (PT) and 20 μg /ml (CT). The ligand concentrations were 10 nM (PAF) and 40 ng/ml (TNF-α). Control: without ligand stimulation and toxin treatment.