FIG. 3.

Inhibition of PAF-induced κB binding activity and HB-EGF gene expression by PDTC. (A) PDTC inhibits PAF-induced κB binding activity. Nuclear protein extracts were prepared from monocytes preincubated for 40 min with PDTC at the indicated concentrations, followed by PAF (100 nM) stimulation for 60 min. The autoradiograph of an EMSA result is shown. (B) PDTC inhibits PAF-induced HB-EGF gene expression. Cells pretreated with PDTC, as indicated above, were stimulated with PAF (100 nM) for 60 min. Total cellular RNA was extracted for Northern blot as described. The autoradiograph of a Northern blot is shown with positions of the ribosomal RNAs indicated